ObjectiveTo explore the necessity of setting up the course on clinical healthcareassociated infection(HAI) and effectiveness of the casebased teaching method in medical higher education. Methods198 students who underwent internship in a hospital between July 2015 and July 2016 were selected, according to whether they selected clinical HAI coures, they were divided into course selective group(n=107, adopted casebased teaching method ) and unselective group(n=91), questionnaire survey on the knowledge and attitude of HAI was carried out. ResultsThe average score on HAI in selective group was higher than that of unselective group(［85.3±1.6］ vs ［58.3±1.8］, t=111.72， P<0.01）. Before and after conducted casebased teaching method, 21.49% and 47.66% of students actively studied respectively, difference was significant (χ2=16.195, P<0.01). ConclusionIt is necessary to carry out a systematic course on HAI, casebased teaching method can improve the learning enthusiasm of students.