ObjectiveTo explore the effect of multidisciplinary team (MDT) combined with bundle management on prevention and control of multidrugresistant organism (MDRO) infection in the intensive care unit(ICU). MethodsPatients who were admitted to the ICU in a tertiary firstclass hospital from January 2013 to December 2015 were studied, MDT combined with bundle management has been applied in the prevention and control of MDRO infection in ICU since January 2014, continuous quality improvement program was performed one year later, isolation of MDROs from specimens of ICU patients before implementation（in the year of 2013）, after implementation（in the year of 2014）, and after continuous quality improvement（in the year of 2015） was compared. ResultsThe infection rates of MDROs in ICU patients before implementation, after implementation, and  after continuous quality improvement were 26.55% (154/580), 17.13% (117/683), and 12.01% (77/641) respectively, showing a downward trend, with a significant difference (χ2=44.030, P<0.001); the total isolation rates of MDROs in ICU patients were 64.44%（154/239）, 63.59%（117/184）, and 43.26%（77/178）respectively，showing a downward trend, with a significant difference （χ2=22.284，P＜0.001）. The main MDROs in ICU were multidrugresistant(MDR) and pandrugresistant(PDR) Acinetobacter baumannii (44.54%). ConclusionMDT combined with bundle management can decrease MDRO infection rate and isolation rate  in ICU.