ObjectiveTo investigate the effect of comprehensive intervention measures on submission of microbiological specimens before therapeutic use of antimicrobial agents. MethodsAugust 2014 to August 2015 was as preintervention group, comprehensive intervention measures were started to carry out from September 2015, September 2015 to September 2016 was as postintervention group. Microbiology specimen submission rates before therapeutic antimicrobial use, positive rate of blood culture, and rate of antimicrobial use in all departments and key departments were compared between preintervention group and postintervention group. ResultsThe submission rates of microbiological specimens before general, restricted, and special therapeutic antimicrobial use increased from 42.21%, 45.19%, and 74.71% of preintervention respectively to 53.54%, 55.68%, and 89.70% of postintervrntion respectively, showing significant difference (all P<0.01); after intervention, except gastrointestinal surgery and trauma department of orthopedics, the other departments all met the requirements of the microbiological specimen submission rates set by the hospital; but submission rates of microbiological specimens from department of gastrointestinal surgery and trauma department of orthopedics increased from 5.46% and 11.67% before intervention to 11.66% and 29.45% respectively after intervention, difference was statistically significant (both P<0.001). The missing report rate of healthcareassociated infection(HAI) dropped from 13.56% before intervention to 10.98% (P< 0.05), and the use rate of antimicrobial agents decreased from 57.36% to 54.47% (P<0.001). ConclusionComprehensive intervention measures can effectively improve the submission rates of microbiological specimens before therapeutic use of antimicrobial agents, reduce missing report rates of HAI and utilization rate of antimicrobial agents, and achieve certain clinical effectiveness.