ObjectiveTo study the effect of chlorhexidine gluconate rubbing bathing on preventing multidrugresistant organism(MDRO) infection in patients in intensive care unit(ICU). Methods108 critically ill patients in a tertiary firstclass hospital between January and December 2016 were randomly divided into trial group and control group. Trial group adopted wet towel containing 2% chlorhexidine gluconate for bathing, control group adopted water for bathing. Bacteriostasis rate, incidence of healthcareassociated infection(HAI), occurrence of MDRO infection, and adverse reaction between two groups of patients after rubbing bathing were compared. ResultsThere was no significant difference in the bacteriostasis rate within 2 hours between two groups（P>0.05）, bacteriostasis rates of trial group after 4, 8, and 24 hours of bathing were significantly higher than control group（P<0.001）. Incidences of HAI in trial group and control group were 44.44%（24/54）and 66.67%（36/54）respectively（P＜0.05）； incidences of MDRO infection in trial group and control group were 20.37%（11/54）and 40.74%（22/54）respectively（P＜0.05）. The main infection sites in trial group and control group were both lower respiratory tract, accounting for 87.50% and 72.22% respectively; 8 cases (33.33%) in trial group and 11 (30.55%) in control group had ventilatorassociated pneumonia (VAP). 11 strains of MDROs in trial group and 22 in control group were isolated, both were mainly carbapenemresistant Acinetobacter baumannii (CRAB). There was no adverse reaction after the bathing in both groups. ConclusionApplication of chlorhexidine gluconate bathing can effectively reduce the incidence of HAI and MDRO infection in ICU patients.