ObjectiveTo analyze the change in isolation rates of multidrugresistant organisms (MDROs) before and after adopting plandocheckact (PDCA) cycle method for management of MDROs. MethodsBacterial culture specimen submission and isolation of MDROs in a tertiary firstclass hospital before the implementation of PDCA cycle (January 2013December 2014) and after implementation of PDCA cycle (January 2015December 2016) were collected and analyzed. ResultsA total of 14 889 specimens were sent for detection before the implementation of PDCA cycle, 6 345 strains were isolated, 650 of which were MDROs, isolation rate of MDROs was 10.24%; after the implementation of PDCA cycle, 17 856 specimens were sent for detection, 7 568 strains were isolated, 476 were MDROs, isolation rate of MDROs was 6.29%; difference in MDRO detection rate before and after the implementation of PDCA was statistically significant （χ2=72.567，P＜0.001）. After CochranArmitage trend test, the isolation rates of MDROs in 20132016 showed a decreased trend (Z=-7.8856). The amount and cost of hand hygiene products have increased. ConclusionBy carrying out PDCA cycle for MDROs management, the isolation rate of MDROs in hospital is reduced. PDCA cycle management method can effectively promote the continuous quality improvement of hospital MDROs management.