ObjectiveTo evaluate the application effect of meticulous management mode on prevention and control of infection related to pharmacy intravenous admixture service（PIVAS）. MethodsQualified detection results of hygiene status of object surface, air culture quality, hand hygiene of medical staff in PIVAS in a hospital from January 2014 to December 2016 were investigated, meticulous management measures were taken to intervene and analyze the detection results.ResultsThe qualified rates of hand hygiene in 2014-2016 were 68.18%, 81.82%, and 100.00% respectively, hand hygiene qualified rates in different years were statistically different（χ2=2.993，P=0.019）. Qualified detection rates of surface of small objects, surface of horizontal laminar flow hoods, surface of biosafety cabinets, and air quality of dressing room I and II in PIVAS all increased to 100% in 2016.ConclusionStrengthening meticulous management of the internal work of PIVAS can effectively improve staff’s standardized operation.