ObjectiveTo understand status of healthcareassociated infection(HAI) and changing trend of pathogens, evaluate the efficacy of HAI management and control measures, and provide basis for improving HAI management level.MethodsCombination of bedside survey and individual case survey was adopted to investigate the prevalence rates of HAI occurred among all hospitalized patients in a tertiary firstclass hospital at a given day in JulyAugust in each year of 2010-2016.ResultsFrom 2010 to 2016, a total of 8 434 patients were surveyed, 419 patients had 476 cases of HAI, HAI rate and HAI case rate were 4.97% and 5.64% respectively，HAI prevalence rates were 3.77%-6.16%，difference was not significant(χ2=10.98，P＞0.05）, prevalence rate of HAI in intensive care unit was 50.91%, mainly was lower respiratory tract infection(51.47%). The top 5 pathogens causing HAI were Acinetobacter baumannii (14.62%), Pseudomonas aeruginosa (14.62%), Klebsiella pneumoniae (13.08%), Escherichia coli (10.38%), and Staphylococcus aureus(7.31%). Antimicrobial usage rate on the survey day was 33.09%, antimicrobial usage rate and antimicrobial prophylaxis decreased from 47.92% and 49.19% in 2010 to 26.62% and 18.26% in 2016 respectively, bacterial culture rate in patients receiving therapeutic antimicrobial use increased from 5.16% in 2010 to 50.00% in 2016, differences were all statistically significant (χ2 =279.44, 265.21, 246.26, respectively, all P＜0.001）.ConclusionIncidences of HAI didn’t change significantly in recent 7 years, usage of antimicrobial agents has been standardized year by year, but pathogenic detection rate in patients receiving therapeutic antimicrobial use needs to be improved.