ObjectiveTo understand the condom use and its influencing factors among female sex workers(FSWs) in Hechi City, provide evidence for formulating prevention and control measures on acquired immunodeficiency syndrome（AIDS）. MethodsAnonymous questionnaire survey was conducted among FSWs in different grades of locations in Hechi City. Chisquare test and KruskalWallis H test were used to analyze status of condom use, influencing factors for condom use were analyzed by logisitic regression analysis. ResultsFrom 2011 to 2015, a total of 10 867 FSWs in Hechi City were surveyed, the total awareness rate of AIDSrelated knowledge was 95.69%, awareness rate of AIDS knowledge enhanced with the increase of location grades (trend χ2=53.72, P<0.01). Analysis on influencing factors for condom use frequency showed that the possibility of condom use in higher grade locations (OR=3.034, 95.0%CI=2.410-3.818) and middle grade locations (OR=1.807, 95.0%CI=1.639-1.992) were higher than that in low grade locations. FSWs with high school and higher education(OR=1.403, 95.0%CI=1.183-1.665) were more likely to use condom than those with junior middle school and lower education. In addition, completely correct response to AIDS knowledge items (OR=2.521, 95.0%CI=2.291-2.773), receiving condom publicity and distribution/AIDS counselling and testing services (OR=2.019, 95.0%CI=1.568-2.599), and receiving peer education (OR=1.538, 95.0%CI=1.365-1.733) were all factors for promoting condom use, while age over 40 years (OR=0.835, 95.0%CI=0.737-0.945) was a risk factor for influencing condom use. ConclusionCondom use is mainly influenced by education degree of surveyed subjects, awareness on AIDSrelated knowledge and so on, strengthening publicity and education on AIDS knowledge as well as performing behavioral intervention in lowgrade locations and middleaged FSWs are beneficial to improving condom use rate.