ObjectiveTo investigate the current status of nurses’ occupational protection during venous blood sampling, and compare whether there are differences in specific occupational protective behaviors among different regions. MethodsAccording to stratification of six major geographical regions of China (East China, South China, West China, North China, Northeast China, Central China), nurses in 12 tertiary firstclass comprehensive hospitals in different regions of China were investigated, a questionnaire survey was performed on organization and management of occupational protection during venous blood sampling, implementation of specific behaviors, and pressing after pulling needles of blood sampling, surveyed results were statistically analyzed, difference among different regions was compared. ResultsA total of 3 176 nurses in 89 departments of 12 hospitals were surveyed. The average number of venous blood sampling by nurses was most in South China(299.88 times), and least in North China (102.03 times). 99.42% (2 910/2 927) of nurses knew the emergency countermeasures for needle stick injuries; only 65.71%（1 901/2 893）of nurses wore gloves while collecting blood, the highest was in West China (98.03%, 398/406), the lowest was in North China（25.00%，115/460）， difference among different regions was statistically significant （χ2=617.32，P＜0.01）； 49.47% （1 445/2 921） of nurses discarded the used needle with one hand, the highest was in Northeast China （66.16%，217/328），the lowest was in East China(40.83%，167/409），difference among different regions was statistically significant（χ2=70.28，P＜0.01）. 65.42%（1 831/2 799）of nurses guided patients to press blood vessel puncture point, the highest was in Central China（74.95%，380/507）， the lowest was in Northeast China(48.73%，154/316)， difference in correct guiding rate of pressing point among different regions was statistically significant （χ2=93.84，P＜0.01）. ConclusionIt is necessary to improve the occupational safety awareness of Chinese nurses during venous blood sampling, implement systematic safety precaution training and standardize occupational protection behaviors, so as to reduce bloodborne pathogen occupational exposure and promote safety of nurses.