ObjectiveTo compare disinfection effect of disinfectant wipes and chlorinecontaining disinfectant, provide basis for improving prevention and control measures for healthcareassociated infection.MethodsAccording to disinfection methods, three groups were divided: Clinell Universal Wipes group(Clinell group), Gelica Surface Disinfecting Towelettes group(Gelica group), and 84disinfectant group, bed units of neonatal ward were cleaned and disinfected, surface sampling of bed units before disinfection, as well as 5, 10, 30 and 60 minutes after disinfection was performed, 30 specimens were taken in each group at each time point, bacterial colony count, bacterial killing rate and qualified rate of disinfection among three groups were compared, methicillinresistant Staphylococcus aureus (MRSA) on object surface was screened.ResultsAfter 5, 10, 30 minute disinfection, there was no significant difference in bacterial colony count and bacterial killing rate of object surface of bed units among three groups(P＞0.05). After 60 minute disinfection, bacterial colony count in Clinell group (［2.61±0.41］CFU/cm2) and Gelica group (［2.71±0.42］CFU/cm2) were both lower than 84disinfectant group(［4.08±0.33］CFU/cm2), difference was significant(P＜0.05)；bacterial killing rate in Clinell group (［72.36±3.90］%) and Gelica group (［71.49±4.77］%) were both higher than 84disinfectant group(［55.92±3.22］%), difference was significant(P＜0.05). The qualified rate of disinfection of bed unit surface in Clinell group was higher than 84disinfectant group(86.67% vs 63.33%, P＜0.05）. All three disinfection methods can effectively remove MRSA from object surface.ConclusionDisinfection effect of disinfectant wipes is reliable, it can effectively kill multidrugresistant organisms on bed unit surface in neonatal ward, and is suitable for routine disinfection in highrisk environment, such as neonatal ward.