ObjectiveTo study the serotype distribution, antimicrobial resistance, and characteristics of extendedspectrum βlactamases (ESBLs) resistance genes of clinically isolated Salmonella in Beijing from 2012 to 2015.MethodsAntimicrobial resistance of 677 Salmonella strains isolated from gastrointestinal clinic in Beijing to 16 kinds of antimicrobial agents was detected by minimal inhibitory concentration method, resistance genes in 244 βlactamase resistance strains were detected by polymerase chain reaction (PCR). Results677 Salmonella strains were divided into 68 serotypes, the top three were Salmonella enteritis, Salmonella typhimurium, and Salmonella senftenberg, antimicrobial resistance of Salmonella to ampicillin and amoxicillinclavulanic acid were 42.54% and 40.77% respectively, 57.16% of strains were resistant to at least 3 kinds of antimicrobial agents. Of 244 strains of ampicillin and amoxicillin/clavulanic acidresistant Salmonella, 174 (71.31%) carried at least one kind of ESBLs gene, 146 were for type blaTEM1, 30 for blaOXA1, and 18 for blaCTXM (7 were for blaCTXM15, 6 blaCTXM55, 5 blaCTXM14); 20 strains harbored two kinds of drug resistance genes at the same time. ConclusionSalmonella in this area has a high carrying rate of ESBLs resistance genes, mainly blaTEM1, accompanied by blaOXA1 and 3 kinds of blaCTXM subtypes, showing a genetic diversity.