ObjectiveTo analyze the change in antimicrobial resistance rate of grampositive bacteria in Shaanxi Provincial Bacterial Resistance Surveillance Network(BRSN) from 2014 to 2016, and provide theoretical basis for treatment of grampositive bacterial infection.MethodsData of grampositive bacteria from participating hospitals in Shaanxi Provincial BRSN between 2014 and 2016 were collected, according to 2016 standards of American Clinical and Laboratory Standards Institute (CLSI), data were analyzed with WHONET 5.6 software.ResultsData of 42 participating hospitals in Shaanxi Provincial BRSN were collected. 43 830, 48 258, and 61 819 strains of bacteria were collected in each year from 2014 to 2016, of which grampositive bacteria were 14 487 (33.1%), 15 303 (31.7%), and 18 613 (30.1%) respectively. The top 5 specimens of grampositive bacteria were sputum (n=14 844, 30.7%), blood (n=8 873, 18.3%), secretion (n=6 271, 13.0%), urine (n=5 225, 10.8%), and wound pus (n=2 449, 5.0%). The top three grampositive bacteria were Staphylococcus spp. (n=28 878, 59.7%), Enterococcus spp. (n=10 207, 21.1%), and Streptococcus spp. (n=8 824, 18.2%). From 2014 to 2016, isolation rates of methicillinresistant Staphylococcus aureus (MRSA) were 36.1%, 45.2%, and 38.6% respectively, isolation rates of methicillinresistant coagulase negative Staphylococcus (MRCNS) were 80.5%, 81.0%, and 83.3% respectively, vancomycin, linezolid, and teicoplaninresistant Staphylococcus spp. were not found; resistance rates of Enterococcus faecalis to vancomycin were 1.7%, 0.8%, and 0.7%, respectively, resistance rates of Enterococcus faecium to vancomycin were 3.1%, 2.0%, and 2.1% respectively; among Streptococcus pneumoniae isolated from noncerebrospinal fluid, isolation rates of penicillinresistant Streptococcus pneumoniae (PRSP) in three years were 11.8%, 6.1%, and 4.7% respectively.ConclusionAntimicrobial resistance of clinically isolated grampositive bacteria is more common in this region, surveillance and management of bacterial resistance should be strengthened, antimicrobial resistance of bacteria should be realized in time, so as to provide basis for rational clinical antimicrobial use.