ObjectiveTo understand status and influencing factors of occupational stress of health care workers(HCWs) in Qingyang City of Gansu Province, so as to provide scientific basis for alleviating occupational stress and improving occupational health of HCWs.MethodsMaslach burnout inventorygeneral survey (MBIGS) and convenient sampling method were adopted to conduct survey on HCWs in city, county, and township levels of hospitals in seven counties and one district of Qingyang City, SPSS 19.0 statistical software was used to analyze the data.ResultsA total of 18 medical institutions were surveyed, and 494 valid questionnaires were collected. Among 494 HCWs, 443 (89.68%) had occupational burnout, 245(49.60%) had moderate or above occupational burnout, at a relatively serious level. The mean scores of three dimensions were: emotional exhaustion （12.65±8.13）, cynicism （6.86±6.09）, and low sense of achievement（22.48±9.03）. The score in emotional exhaustion of HCWs with years of service≤5 years was lower than that of 5-10 years group, difference were both significant (P<0.05); and score in cynicism of HCWs with years of service≤5 years was lower than that of 5-10 and 10-15 years, difference were both significant (both P<0.05). The scores in low sense of achievement of HCWs with master degree or higher were lower than those of undergraduate, junior college or lower, difference was significant (P<0.05). The scores in low sense of achievement of HCWs with high professional title were higher than those of associate, middle, and primary groups, difference was significant (P<0.05). There was no significant difference in the scores of emotional exhaustion, cynicism, and low sense of achievement among HCWs of different gender, occupation, and marital status (all P>0.05). ConclusionOccupational stress of HCWs in Qingyang City of Gansu Province is serious, management department can take targeted intervention measures to alleviate occupational stress of HCWs with long length of service year, high educational level, and low professional title, protect occupational health of HCWs, and improve level of medical service.