ObjectiveTo explore scientific and effective management methods for safe injection, provide reference for improving compliance rate of safe injection behavior and achieving continuous improvement in safe injection.MethodsA special safety injection program was formulated, baseline survey and safe injectionrelated training were carried out, safe injection system and standard procedures were formulated, onsite supervision and examination were strengthened, quantitative indexes such as qualified rate of safe injection facilities, theoretical examination result, behavior compliance rate, and occurrence rate of sharp injury before intervention (baseline survey in May 2016) and after intervention (2017) were compared.ResultsThrough comprehensive intervention, facilities allocation rates of safe injection in whole hospital were all>97%, qualified rates of all medical waste disposal were all>93%, actual average consumption of alcoholbased hand rub in the whole hospital increased from (5.56±2.13)  mL/bedday to (9.95±5.38)mL/bedday; the average score of safety injection knowledge examination of health care workers(HCWs) increased from (71.20±12.22) before intervention to (92.59±5.99) after intervention; HCWs’ compliance rate to safe injection increased from 62.50% before intervention to 88.53% after intervention, difference were all statistically significant (P<0.05). Reporting rate of sharp injuries in the whole hospital within a week increased from 15.79% before intervention to 71.43% after intervention; occurrence rate of case incidence of sharp injuries in the whole year and 100day hospitalization decreased from 9.98% and 0.0276% before intervention to 5.31% and 0.0168% after intervention respectively, differences were both statistically significant (both P<0.05).ConclusionEffective comprehensive intervention measures can strengthen the awareness of safe injection, improve compliance rate of safe injection behavior, effectively prevent the occurrence of occupational injuries in HCWs caused by unsafe injection, and prevent the spread of healthcareassociated infection.