ObjectiveTo understand healthcareassociated infection(HAI) rate and hand hygiene compliance rate in a hospital during Joint Commission International(JCI) accreditation stage, and evaluate the implementation efficacy of HAI control measures based on JCI accreditation standards.MethodsThree groups were divided according to preimplementation (preaccreditation), initiation (periaccreditation), and postaccreditation (postaccreditation) of JCI standards, difference in hand hygiene compliance rate, HAI rate, and consumption of hand hygiene products among health care workers(HCWs) at different stages were compared.ResultsCompliance rate of hand hygiene among all HCWs increased from 74.99% before JCI accreditation to 78.61% during periaccreditation period, and then to 90.95% after the accreditation, the overall difference was statistically significant (P<0.001). After JCI accreditation, consumption of hand sanitizer and alcoholbased hand rub increased in all wards of the hospital, HAI rates in departments of cardiology and oncologyhematology as well as the whole hospital decreased (P<0.05), and HAI rates also showed a downward trend (P<0.05). Compliance rate of HCWs’ hand hygiene was negatively correlated with HAI rate (r=-0.99, P<0.05).ConclusionAccording to JCI accreditation standards, realtime improvement can effectively improve hand hygiene compliance rate of HCWs and reduce HAI rate.