ObjectiveTo understand the risk factors for methicillinresistant Staphylococcus aureus (MRSA) infection in intensive care unit(ICU) patients. Methods825 ICU patients in a tertiary general hospital from 2014 to 2016 were selected, a casecontrol study was conducted, patients infected with MRSA 48 hours after ICU admission were in case group, and those without MRSA infection 48 hours after ICU admission were in control group, univariate and multivariate logistic analysis were conducted.ResultsAmong 825 patients, 64(7.76%) had MRSA infection. The major MRSA infection was pulmonary infection(45.31%), followed by skin and soft tissue infection (18.75%), surgical site infection(9.38%), bloodstream infection (9.38%), intracranial infection(6.25%）, abdominal infection (6.25%), and thoracic infection (4.68%). Multivariate logistic regression analysis showed that independent risk factors for MRSA infection in ICU patients were surgery within a month （OR95%CI：2.628-9.166）, trauma (OR95%CI：2.248-9.280）, duration of tracheal intubation>7 days (OR95%CI：1.651-11.746), coma (OR95%CI：1.813-8.334）, longterm bedridden (OR95%CI：1.362-10.127）, readmission to ICU (OR95%CI：1.475-7.915）, parenteral nutrition (OR95%CI：1.521-7.518）, use two or more antimicrobial agents (OR95%CI：1.523-6.132）, and multiple organ failure (OR95%CI：1.024-3.948）(all P<0.05).ConclusionPrevention and control of MRSA in highrisk patients such as tracheal intubation, multiple organ failure, coma, longterm bedridden, and trauma should be paid attention, patients should be transferred out of ICU as soon as possible if their condition is permitted.