Objective To understand drug resistance mechanism and clinical characteristics of linezolid-resistant Staphylococcus capitis(LRSC). Methods LRSC isolated from a hospital was collected, homology of the strains was analyzed by pulsed-field gel electrophoresis(PFGE), 23S rRNA gene, rplC and rplD genes which encode ribosomal protein L3 and L4, as well as drug-resistant gene CFR were amplified by polymerase chain reaction(PCR), then sequenced. Results Seven strains of LRSC were isolated from specimens of 5 patients, strains were resistant to oxacillin, levofloxacin, gentamicin and other commonly used antimicrobial agents, only susceptible to glycopeptides, rifampicin, tetracycline and quinupristin/dalfopristin. 23S rRNA mutation were found in all 7 drug-resistant strains, all strains didn't carry CFR gene. PFGE showed that 7 clinical strains belonged to the same genotype. All 5 patients had serious underlying diseases, length of hospital stay of patients who isolated drug-resistant bacteria was 92 days to 10 months, 3 of whom indwelled deep venous catheters, 1 patient indwelled both deep venous catheter and dialysis catheter, and 3 patients received linezolid treatment. Conclusion Resistance of 7 strains of S. capitis to linezolid is due to G2576T and C2104T mutations in V region of 23S rRNA; LRSC showed multidrug-resistant phenotype, factors for drug-resistant bacterial infection may be patients with underlying diseases, prolonged length of hospital stay, long course of treatment with linezolid and indwelling deep vein catheter.