Objective To study the disinfection efficacy of hypochlorite disinfectant on fiberoptic bronchoscope by automatic soft endoscope cleaning-disinfection machine. Methods Seven fiberoptic bronchoscopes used in a hospital in June-July 2018 (total 48 days) were monitored. Fiberoptic bronchoscopes were cleaned and disinfected with hypochlorite disinfectant by machine for 5 minutes, ATP fluorescence detection method, filter membrane method and pouring method were used to monitor and evaluate the disinfection efficacy on the outer surface and inner surface of fiberoptic bronchoscopes. Results Seven fiberoptic bronchoscopes were sent to central sterile supply room for 159 times of cleaning and disinfection. Specimens were taken 30 times in different dates, surface cleanliness was detected by ATP fluorescence method with RLU range of 10-64 (median was 22), and qualified rate was 100%. A total of 159 times of detection were performed on the inner surface, colony counts were both 0-6 CFU/piece detected by filter membrane method and pouring method, and qualified rates were both 100%. The results of seven cycles of stability test showed that the initial concentration of available chlorine was 210 mg/L when hypochlorite disinfectant was used in automatic endoscope cleaning-disinfection machine, after one-week disinfection, endoscopes were treated 20-32 times, and concentration of available chlorine was 74-106 mg/L. Conclusion Hypochlorite disinfectant can meet the prescribed disinfection requirements when it is used for disinfection of fiberoptic bronchoscope by automatic soft endoscopy cleaning-disinfection machine, the effective concentration of hypochlorite disinfectant can still be maintained when it is used in machine for 7 days.