Objective To investigate the management status of central sterile supply departments (CSSDs) in secondary and tertiary hospitals in Shanghai, put forward scientific basis and reference for promoting further implementation of WS 310.1-2016 Hospital Central Sterile Supply Department- Part 1:Management Standard. Methods According to the requirement of WS 310.1-2016, self-designed questionnaires were used to investigate the management of CSSDs of 58 hospitals in 16 districts and counties of Shanghai. Results 94.83% of hospital CSSDs implemented centralized management, 85.19% of hospital CSSDs undertook cleaning and disinfection management of loaner medical devices and implants, there was no significant difference in the implementation rate between secondary and tertiary hospitals (all P>0.05). 70.37% of hospital CSSDs completed monitoring management on the initial sterilization of loaner medical devices and implants, tertiary hospitals performed better than secondary hospitals(χ²=5.684,P<0.05). 48.28% of hospital CSSDs equipped with insulation detector, equipping rate in tertiary hospitals was higher than secondary hospitals(χ²=5.557,P<0.05). Conclusion The overall situation of implementing WS310.1-2016 in secondary and tertiary hospitals in Shanghai is good, but disposal and management of loaner medical devices and implants by CSSDs need to be improved urgently, and equipping of insulation detector should be paid attention.