Objective To explore disinfection effect of one kind of bedding cleaning technology on cotton quilts, mattresses and so on. Methods From November 2018 to January 2019, partial cotton quilts, mattresses and pillow cores were randomly selected from the departments of neurosurgery, general surgery and neurology in a hospital, one kind of bedding cleaning technology was used to disinfect them, difference in bacterial colony after 10-day and 30-day use as well as before and after cleaning were compared. Results Cotton quilts, mattresses and pillow cores of 72 bed units were selected from three departments. After cleaned and disinfected with a kind of bedding cleaning technology, coliform bacteria and Staphylococcus aureus were not detected on cotton quilts, mattresses and pillow cores, bacterial contamination rates of cotton quilts and mattresses in department of general surgery were both 4.2%, bacterial colonies on cotton quilts, mattresses and pillow cores in other departments were all qualified. Bacterial colonies on cotton quilts, mattresses and pillow cores used by patients in departments of neurosurgery and neurology for 10 days and 30 days were both significantly different from those after cleaning and disinfection(all P<0.01); bacterial colonies on cotton quilts used by patients in departments of general surgery for 10 days and 30 days were not significantly different from those after cleaning and disinfection(both P>0.05); but bacterial colonies on mattresses and pillow cores used by patients for 10 days and 30 days were both significantly different from those after cleaning and disinfection(both P<0.01). Conclusion Disinfection effect of this kind of bedding cleaning technology on cotton quilts, mattresses and so on is reliable, which can prevent the occurrence of healthcare-associated infection caused by contamination of cotton quilts, mattresses and pillow cores.