Objective To investigate the current situation of disinfection of high-touched object surface in neurosurgical intensive care unit (ICU), and explore the optimal frequency of disinfection. Methods Six kinds of high-touched objects, including bed bar, micro-pump, monitor button, hand sanitizer button, mouse and medical history, were selected as the research objects in neurosurgical ICU of The First Hospital of Jilin University. Specimens were taken at 2, 4, 6 and 8 hours after disinfection, and colony counts were calculated after 48 hours of culture. The number of colonies at different time points after disinfection were compared. Results There was no significant difference in the numbers of colonies on bed bar and monitor button at different sampling time points after disinfection(all P>0.05), the average number of colonies was<5 CFU/cm². There were significant differences in the numbers of colonies on micro-pump, hand sanitizer button, mouse and medical history at different sampling time points after disinfection (all P<0.05); the number of colonies at 8 hours after disinfection were all higher than that at 6 hours after disinfection (all P<0.05). Conclusion There are some differences in surface contamination of different high-touched object surface in ICU, the best disinfection frequency should be determined according to the actual condition of high-touched object surface.