Objective To explore the value of modified Hodge test (MHT), Carba NP test (CNPt) and modified carbapenem inactivation test (mCIM) in detecting carbapenemase-producing Klebsiella pneumoniae(K. pneumoniae). Methods 117 clinically isolated strains of K. pneumoniae were collected from a hospital between December 2016 and November 2017, all strains were performed antimicrobial susceptibility testing, 57 strains were carbapenem -resistant and 60 strains were carbapenem-susceptible. Carbapenemase gene detected by PCR was as gold standard, value of three methods for detecting carbapenemase in K. pneumoniae was evaluated. Results Of 57 carbapenem-resistant K. pneumoniae strains, 40 were positive for PCR, including 39 KPC strains and 1 NDM-1 strain, no other resistance genes were detected. Positive rates of MHT, CNPt, and mCIM were 87.7% (50/57), 89.5% (51/57), and 91.2% (52/57) respectively. No carbapenemase gene was detected among 60 strains of susceptible bacteria by PCR, results of three phenotypic screening tests were all negative. Taking PCR as the gold standard, sensitivity of MHT, CNPt, and mCIM were 97.5% (39/40), 100% (40/40), and 100% (40/40) respectively, specificity were 85.7% (66/77), 85.7% (66/77), and 84.4% (65/77) respectively. Conclusion CNPt is a reliable phenotypic screening method, which can be used in epidemiology and healthcare-associated infection surveillance. mCIM has the advantages of simple operation, easy to make a judgment and is more suitable for clinical microbiology laboratory to carry out routinely.