Objective To identify risk factors for multidrug-resistant organism(MDRO) infection in patients with diabetic foot ulcer(DFU) by Meta-analysis. Methods Literatures on risk factors for MDRO infection in patients with DFU were retrieved and screened according to inclusion and exclusion criteria, data were extracted and performed quality assessment independently by two researchers, Meta-analysis was performed using RevMan 5.3 statistical analysis software. Results A total of 20 articles were included, with a total samples of 2 568 cases. There were 13 risk factors were included in the analysis, Meta analysis showed that risk factors for MDRO infection were course of foot ulcer (WMD, 5.25[95%CI, 0.13-10.37], P=0.04), ischemic ulcer (OR, 2.84[95%CI, 2.25-3.60], P<0.001), ulcer area (OR, 2.43[95%CI, 1.58-3.72], P<0.001), number of hospitalization due to the same ulcer>2 times(OR, 16.82[95%CI, 10.85-26.09], P<0.001), history of antimicrobial exposure(OR, 5.86[95%CI, 3.79-9.07], P<0.001), use of third generation cephalosporins (OR, 5.15[95%CI, 3.51-7.56], P<0.001), complicated osteomyelitis(OR, 7.22[95%CI, 4.45-11.69], P<0.001), diabetic nephropathy(OR, 1.41[95%CI, 1.12-1.76], P=0.003), and anemia(OR, 3.18[95%CI, 2.04-4.95], P<0.001). Conclusion Risk of MDRO infection is higher in DFU patients with long course of foot ulcer, ischemic ulcer, larger area of ulcer, the more times of hospitalization, history of antimicrobial use, combined osteomyelitis, diabetic nephropathy, and anemia, health care workers should identify relevant risk factors in time and take active measures to prevent the occurrence of MDRO infection.