Objective To compare the terminal disinfection effect of different dosage of hydrogen peroxide vapor (HPV) on burn intensive care unit(BICU). Methods A 110 m³ empty ward in department of burn and plastic surgery of a hospital was selected as the test location. Bacillus subtilis var. niger spore tablets were placed at 15 points without shielding (0, 1, 2, 3, 4 m away from the disinfector, each point was divided into upper, middle and lower planes) and 15 points (same position) in the uncovered sterile test tube, hydrogen peroxide indicator tape was placed at each point of uncovered sterile test tube and non-shielding point. The amount of hydrogen peroxide was calculated according to the size of ward (8 g/m³ and 12 g/m³, respectively). After 5 hours of disinfection, change in color of chemical indicator tape was observed, spore tablets were placed in broth culture tube, result was observed after culture. Data was analyzed using SPSS 20.0 statistical software. Results When 8 g/m³ hydrogen pero-xide was used for disinfection, the peak concentration was 255.9 g, the time to reach peak concentration was 1 hour and 48 minutes; when 12 g/m³ hydrogen peroxide was used for disinfection, the peak concentration was 337.6 g, the time to reach peak concentration was 2 hour and 26 minutes. When disinfection time of two disinfection dosages reached 8 hours, the concentration of ward was basically the same. Under the condition of non-shielding, chemical indicator tape of two kinds of disinfection dosage all changed color, but could not completely change color in uncovered sterile test tube. When disinfection dosage of hydrogen peroxide was 8 g/m³ and 12 g/m³, positive rates of the non-shielding specimens were 6.67% and 2.67% respectively, with no significant difference (P>0.05), and disinfection effect was not significantly different among different points (all P>0.05). Conclusion Change in color of chemical indicator tape can be used as a preliminary evaluation of disinfection effect in clinic. When using HPV for terminal disinfection, it is necessary to expose the items as far as possible, disinfection time and dosage should be determined according to the actual situation, disinfection effect of different points in the ward is basically the same.