Objective To explore the application efficacy of plan-do-check-act (PDCA) cycle in hospital environmental hygiene supervision and management. Methods PDCA tools were adopted to supervise and manage hospital environmental hygiene, isolation rates of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resis-tant(MDR) Acinetobacter, as well as qualified rate of ATP fluorescence detection before and after the implementation of supervision mode were compared. Results After the implementation of PDCA cycle supervision mode, isolation rate of MDR Acinetobacter, overall isolation rate of MRSA and MDR Acinetobacter on the surface of environmental objects in two departments were both lower than those before intervention (0.96% vs 8.04%; 6.73% vs 15.18%, respectively), with statistical significance (both P<0.05). Before intervention, a total of 17 strains of MDROs were isolated, 7 strains of MDROs were isolated from bedside instruments, and 2 strains were isolated from ground, dairy appliances and ward bed respectively; after intervention, a total of 7 strains of MDROs were isolated, 2 strains were isolated from bedside instruments and ground respectively. Qualified rate of ATP detection on environmental surface after intervention was higher than before intervention (82.61%[38/46] vs 58.33%[28/48], χ²=8.451,P=0.004). Conclusion Applying PDCA cycle supervision mode, active finding out problems and strictly controlling process management can effectively reduce the isolation rates of MRSA and MDR Acinetobacter on the surface of hospital environmental objects, ensure medical environmental hygiene and maintain medical safety.