Objective To analyze the clinical characteristics, pathogenic species and drug resistance of patients with hospital-acquired candidemia(HAC), and explore risk factors affecting the prognosis of patients with candidemia. Methods HAC cases which were confirmed by blood culture in Nanfang Hospital of Nanfang Medical University between January 1, 2008 and December 31, 2018 were chosen, demographic and clinical data were collected, patients were divided into survival group and death group according to the prognosis, statistical analysis was performed. Results Clinical data of 240 patients with HAC were collected, including 155 males and 85 females, with an average age of (50.4±16.6) years. 90 strains (37.5%) of Candida albicans (C. albicans), 63 strains (26.3%) of C. parapsilosis and 53 strains (22.1%) of C. tropicalis were isolated from blood culture. Susceptibility rates of C. albicans and C. parapsilosis to azoles were more than 90%, susceptibility rates of C. krusei, C. glabrata and C. tropicalis to fluconazole were all low (40.0%, 59.3%, 62.3%, respectively), susceptibility rates of C. tropicalis and C. krusei to itraconazole were also low(62.3%, 60.0%, respectively). Multivariate analysis showed that organ failure (OR=5.872, P<0.001) and septic shock (OR=3.313, P=0.005) were independent risk factors for the death of patients with HAC. Conclusion C. albicans is the most common pathogens causing candidemia, Candida spp. is detected to be resistant to fluconazole, flucytosine and itraconazole, patients with candidemia have multiple underlying diseases, serious illness and high mortality, organ failure and septic shock are independent risk factors for the death of patients with candidemia.