Objective To evaluate changes of main performance indicators of surgical masks after simulated reuse cleaning and disinfection. Methods Particle filtration efficiency (PFE) and bacterial filtration efficiency (BFE) were used as the key performance indicators of surgical masks, experimental conditions such as different wearing time, different drying methods as well as different washing methods were verified. Results The key performance indicators of surgical mask after experiment met the standard requirements. Conclusion After water washing, 56℃ hot water immersion and 100℃ boiling water immersion, the main performance indicators of surgical masks don't decrease significantly.