Coronavirus disease 2019(COVID-19) has a strong impact on global health safety. As direct contacts of COVID-19 patients, health care workers(HCWs) in isolation ward area are faced with risk factors of occupational injury, including biological, physical, chemical and psychological factors. How to conduct occupational safety protection for HCWs in isolation ward area, and make HCWs fell confident and competent in implementing timely and effective treatment for patients is an urgent problem for medical team to be solved. According to the working experience of epidemic area in Wuhan,in this paper, risk factors of occupational injury of HCWs in isolation ward area were analyzed, the step-down training mode of occupational safety protection was established, personal protective equipment guidance post was set up, emergency plan for occupational exposure and physical discomfort was deve-loped, aiming at ensuring occupational safety of HCWs in COVID-19 isolation ward area, eliminating fear, and enhancing work confidence.