Pneumonia caused by novel coronavirus infection is an emerging infectious disease, prevention and control of healthcare-associated infection (HAI) is a very important work, because hospitals are places for confirmed and suspected patients. Due to the psychological pressure of sudden epidemic situation and unfamiliarity with the characteristics of new diseases, health care workers are faced with a lot of confusion in the prevention and control of HAI. This paper discusses the the common confusion on the aspects of health care workers protection, hand hygiene, isolation requirements and so on.