Objective To systematically assess risk factors for healthcare-associated infection(HAI) with Stenotrophomonas maltophilia (SMA). Methods Literatures about the risk factors for SMA HAI were retrieved from PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure(CNKI), Chinese Biology Medicine(CBM) database, Wanfang database (Wanfang), and VIP database from the establishment of database to March of 2019, literatures were screened, extracted, and performed quality evaluation according to inclusion and exclusion criteria by two researchers, Meta-analysis was conducted using RevMan 5.3 software. Results A total of 10 literatures were included in study, 3 in English and 7 in Chinese. Meta-analysis showed that APCHE-II score>20 at admission (OR=3.69, 95%CI:1.58-8.59), length of ICU stay>7 days (OR=2.14, 95%CI:1.55-2.95), length of hospital stay (MD=7.54, 95%CI:6.22-8.86), use glucocorticoid (OR=2.40, 95% CI:1.57-3.68), combined with immunodeficiency disease (OR=2.31, 95%CI:1.64-3.25), mechanical ventilation (OR=7.59, 95%CI:3.40-11.97), tracheal intubation (OR=4.98, 95%CI:3.21-7.73), tracheotomy (OR=4.31, 95%CI:2.80-6.64), central venous catheterization (OR=1.68, 95%CI:1.19-2.38), indwelling nasogastric tube (OR=3.10, 95%CI:1.83-5.26), use carbapenems (OR=2.81, 95%CI:2.24-3.54), use β-lactamase inhibitors (OR=3.52, 95%CI:1.71-7.24), use aminoglycosides (OR=1.93, 95%CI:1.37-2.73), use glycopeptides (OR=3.42, 95%CI:1.13-10.32) and use antimicrobial agents ≥ 3 kinds(OR=2.70, 95%CI:1.75-4.16) were risk factors for SMA HAI (all P<0.05). Conclusion There are many risk factors for SMA HAI, targeted preventive measures should be taken in clinical practice to reduce the risk of infection.