Objective To explore the drying effect of different drying methods on flexible endoscope and its accessories with lumen, and provide basis for clinical selection of better drying methods. Methods 600 choledochoscopes and accessories with lumen to be dried were selected and randomly divided into three groups with 200 devices in each group:group A:wipping the device surface with 95% ethanol-based lint-free fabric, using syringe to pump 95% ethanol into the lumen of device with opening at both ends, drying water on the surface and lumen of device with a pressure air gun; group B:wiping water on device surface one by one with a dry lint-free fiber, then blew the lumen with a pressure air gun for 3-5 seconds, and put it into a low-temperature vacuum drying cabinet at 50℃ for 20-25 minutes; group C:blow lumen with a pressure air gun for 3-5 seconds, put it in a high-temperature drying cabi-net at 60-65℃ for 10-12 minutes, and then put it in a low-temperature vacuum drying cabinet at 50℃ for 20-25 minutes. Determine the drying effect by visual inspection+air gun blowing+weighing, and evaluate the working efficiency by measuring the drying time. Results The qualified rates of drying device in group A, B and C were 86.00%, 94.00% and 98.00% respectively, difference was statistically significant (P<0.05), the drying effect of group B and C was better than that of group A (P<0.0175), difference between group B and C was not statistically significant (P=0.041). The drying time required for surface drying in group A, B and C were 15, 20 and 12 minutes respectively; the drying time required for lumen in group A, B and C with openings at both ends were 1, 20 and 20 minutes respectively, group A could not handle device with blind end, group B and C needed 25 minutes for blind end. Conclusion For flexible endoscope and accessories with lumen which can not endure high temperature, use pressure air gun to blow the lumen for 3-5 seconds, put into high-temperature drying cabinet at 60-65℃ for 12 minutes, then put into low-temperature vacuum drying cabinet at 50℃ for 20-25 minutes, the drying and disinfection effect is the best, and the working efficiency is the highest.