Objective To optimize the allocation of personal protective equipment (PPE) and disinfection supplies for dispatched medical teams under the extreme shortage of PPE and disinfection supplies for the prevention and control of coronavirus disease 2019 (COVID-19), and ensure a balanced allocation of PPE and disinfectant supplies between the hospital and their dispatched medical team members. Methods 85 members in 7 batches of medical teams dispatched from a tertiary first-class hospital in Shenyang City to different cities during prevention and control period of COVID-19 epidemic were surveyed. The initial allocation plan of PPE and disinfection supplies for medical team was determined firstly, WeChat application program was used, questionnaire for the actual consumption of each initially allocated PPE and disinfection supplies was designed, medical team members were surveyed by electronic questionnaire through WeChat. Results 80 available questionnaires were obtained, 38.75% (n=31) assisted hospitals of the surveyed team members could provide PPE and disinfection supplies as required, 50.00% of which could provide PPE as required, 57.50% could provide disinfection supplies as required, only 38.75% of the assisted hospitals could fully provide PPE and disinfection supplies, 3.75% of the assisted hospitals couldn't provide PPE and disinfection supplies. The total consumption and initial allocation of surgical mask, latex glove, long latex glove, medical goggles, pail, packaged sanitary and disinfection wipes, individually packed sanitary and disinfection wipes, cleaned gauze, alcohol-based hand rub(100 mL/bottle), and effervescent chlorine disinfectant tablet in three assisted areas were all statistically different(all P<0.05). Consumption of surgical mask, medical protective clo-thing, long latex glove, medical goggles, face shield, non-joined work clothes, white coat, work shoes, and pail in different assisted areas were all significantly different(all P<0.05). Conclusion The initial minimum allocation plan of protective and disinfection supplies was based on high-risk occupational exposure and full work load of 8 hours a day, which can meet the actual needs of dispatched team members at the early stage, there is no obvious deviation between the expected plan and actual demand, the allocation of protection resources between hospitals and dispatched medical team members during the period of shortage of PPE and disinfection supplies is balanced, it is the basis and key to ensure the effective implementation of disinfection, isolation and protection measures by both hospital and medical team members.