In order to better prevent novel coronavirus (2019-nCoV) healthcare-associated infection, ensure the safety of health care workers(HCWs) and other staff in medical institutions, and rationally use personal protective equipment(PPE), Healthcare-associated Infection Control Branch of Chinese Preventive Medicine Association, Healthcare-associated Infection Management Committee of Chinese Hospital Association, and Chinese Journal of Infection Control jointly organize domestic experts (including some experts on the frontline of anti-epidemic in Wuhan) to jointly formulate this consensus. This consensus is mainly applicable for medical institutions which receive and cure both patients with coronavirus disease 2019 (COVID-19) and common patients; guide frontline HCWs, non-frontline HCWs, and other staff in medical institutions to rationally and effectively use protective equipment. This consensus determines personal protection standards of staff at different locations and with different working posts through assessment on risk of exposure to COVID-19 among staff in medical institutions. The main contents of this consensus also include introduction and application scope of PPE, putting on and taking off process of three level protection, prevention and emergency treatment of abnormal PPE, treatment process of occupational exposure to COVID-19. At the same time, this consensus particularly emphasizes the rational division of medical institutions during COVID-19 epidemic period, arrange experienced clinicians of departments of infectious or respiratory diseases participate in triage at key triage location, which are the prerequisite requirements of risk assessment; for the selection of PPE, we should not only prevent inadequate protection, but also avoid abuse and overuse, so as to prevent HAI on the premise of ensuring the safety of staff.