During the epidemic period of coronavirus disease 2019(COVID-19) outbreak, how to carry out the prevention and control work quickly, orderly, systematically and with high-quality is a huge challenge facing the general hospital. In accordance with relevant national guidelines and specifications, combined with the actual situation and previous experience of the hospital, Xiangya Hospital of Central South University has formulated a scientific prevention and control plan, issued strong prevention and control measures, and achieved the goal of zero infection of front-line health care workers, zero spread of hospital epidemic situation, and zero death of confirmed patients in the hospital. This paper introduces the practice and experience of Xiangya Hospital of Central South University in the prevention and control COVID-19.