Objective To investigate effect of sterile therapeutic towel wrapping connector and three-way tube of central venous catheter(CVC) on reducing incidence of central line-associated bloodstream infection (CLABSI) in intensive care unit (ICU) patients. Methods Patients with central vein catheterization in surgical ICU of a tertiary first-class hospital in Sichuan Province were selected as the study objects, patients who were admitted to ICU from May to October 2017 were as control group, those from May to October 2018 were as trial group. In control group, routine nursing measures of deep vein catheterization were carried out, in trial group, on the basis of routine nursing measures, CVC and three-way tube were wrapped with sterile therapeutic towel and replaced every 8 hours. Incidence of CLABSI was compared between two groups of patients, self-designed questionnaire was used to investigate the feeling of clinical nurses half a year after the implementation of measures. Results 188 patients in control group and 166 patients in trial group were included in study. In control group, 7 patients developed CLABSI, incidence of CLABSI was 3.72%, central vein catheterization days was 1 233 days, incidence was 5.68 per 1 000 catheter days; in trial group, 5 patients developed CLABSI, incidence of CLABSI was 3.01%, central vein catheterization days was 1 098 days, incidence was 4.55 per 1 000 catheter days; there was no significant difference in the incidence of CLABSI between two groups of patients(χ²=0.136,P=0.712). A total of 65 nurses were investigated about their feelings of clinical use of sterile therapeutic towel wrapping connector and three-way tube of CVC, 98.46% of nurses thought it was inconvenient to open and bandage the sterile therapeutic towel when adding medicine, 93.85% thought it was not conducive to observing the connection of catheter, 66.15% encountered the problems of untimely observation when catheter connection was loose and liquid was oozing out due to the wrapping of sterile therapeutic towel, 53.85% thought that it took about 2-3 minutes to wrap the sterile therapeutic towel for each patient. Conclusion It can not be proved that sterile therapeutic towel wrapping collector and three-way tube can reduce the incidence of CLABSI, while greatly increase the clinical nursing workload, reduce the time of nurses to directly care for patients, and increase the risk of clinical nursing.