Objective To explore the methods of prevention and control of pertussis in neonates. Methods Clinical data of 8 neonates with pertussis in a hospital were collected, general condition, clinical manifestations, auxiliary examination and treatment were analyzed. Results Only 4 of 8 neonates with pertussis had a definite history of contact with patients with respiratory tract infection, the average time from mild cough to spasmodic cough was(6.25±1.91) days, 3 cases (37.50%) had dyspnea during spasmodic cough period, 4 (50.00%) had carbon dioxide retention, 2 (25.00%) had apnea, 6 (75.00%) were with decrease transcutaneous oxygen; 7 neonates with pertussis for the first times showed leukocytes>20×10⁹/L, lymphocytes>60%, procalcitonin<0.5 ng/mL, polymerase chain reaction of Bordetella pertussis were all positive, and pertussis IgM antibody were all negative. Bordetella pertussis isolated from 8 neonates with pertussis were sensitive to azithromycin, 7 neonates was improved and cured, 1 neonate gave up treatment. Conclusion In the epidemic season of pertussis, it is forbidden to visit the neonate. Neonatal pertussis should be diagnosed as early as possible, treated actively and controlled epidemic according to the clinical manifestations and auxiliary examination.