Transmission of antimicrobial-resistant organisms(AROs) is a serious threat to human health, currently, the problem of antimicrobial resistance is becoming more and more serious, causing a great burden on medical and health care. Strengthening the prevention, control, diagnosis and treatment of AROs infection is an important part of the prevention and control of AROs infection in medical institutions. This consensus analyzes the current situation of epidemiology, antimicrobial resistance mechanism and laboratory detection of clinically important AROs, and puts forward expert recommendations on the prevention and control of transmission of AROs infection, aiming to improve the awareness of prevention and control, standardize the prevention and control strategy of transmission of clinically important AROs infection, and clarify the prevention and control process.