Objective To understand the change in antimicrobial resistance of clinical non-fermentative Gram-negative bacilli in China. Methods Surveillance on antimicrobial resistance was carried out according to the requirements of China Antimicrobial Resistance Surveillance System(CARSS), referring to Clinical & Laboratory Stan-dards Institute(CLSI) standard, clinical data of non-fermentative Gram-negative bacilli reported to CARSS by more than 1 000 hospitals in China from 2014 to 2019 were summarized and analyzed. Results Non-fermentative Gram-negative bacilli were mainly isolated from respiratory tract specimens(sputum and bronchoalveolar lavage fluid), urine, pus, and blood, accounting for 83.6%, 6.5%, 4.0% and 3.6% respectively. Isolation rates of Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia, and Burkholderia cepacia were 8.8%, 7.3%, 2.1% and 0.5% respectively. Susceptibility rates of Pseudomonas aeruginosa to polymyxin B, amikacin and gentamicin were 96.9%-98.2%, 89.1%-94.0% and 80.1%-86.4% respectively; susceptibility rates to piperacillin and aztreonam were 66.7%-72.8% and 58.8%-62.4% respectively; susceptibility rates to other antimicrobial agents were 70.4%-81.7%. Susceptibility rates of Acinetobacter baumannii to polymyxin B, amikacin and minocycline were 96.2%-98.0%, 51.0%-58.0% and 59.4%-63.4% respectively, to other antimicrobial agents were 35.8%-50.0%. Susceptibility rates of Stenotrophomonas maltophilia to minocycline, sulfamethoxazole and levofloxacin were 93.0%-95.3%, 89.3%-91.4% and 85.0%-86.6% respectively, susceptibility rates to ticarcillin/clavulanic acid and chloramphenicol were 37.4%-50.3% and 46.6%-51.1% respectively. Susceptibility rates of Burkholderia cepacia to ticarcillin/clavulanic acid and chloramphenicol were 18.5%-37.0% and 54.4%-62.2% respectively, susceptibility rates to other antimicrobial agents maintained 64.7%-86.8%. Isolation rate of carbapenem-resistant Pseudomonas aeruginosa decreased, while isolation rate of carbapenem-resis-tant Acinetobacter baumannii increased first and then decreased. Conclusion There is no significant change in susceptibility of non-fermentative Gram-negative bacilli to commonly used antimicrobial agents, isolation rates of carbapenem-resistant Pseudomonas aeruginosa and carbapenem-resistant Acinetobacter baumannii showed a downward trend.