Objective To explore the prevalence clone and molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) in China, provide practical data support for clinical trace as well as real-time monitoring on prevention and control of CRKP healthcare-associated infection. Methods Literatures related to CRKP strains in China from 2017 to 2019 were collected through literature retrieval, places where strains could be collected were included in analysis, literatures on drug resistance genes detected with polymerase chain reaction (PCR) and sequence type (ST) detected with multilocus sequence typing (MLST) were analyzed, BioNumerics was used to create minimum spanning tree. Results A total of 40 literatures were included in analysis, involving 2 094 CRKP strains, these strains distributed in 15 provinces and cities in 6 regions (northeast, north, east, south, central and southwest China), 1 631 (77.89%) CRKP strains carried KPC resistance genes; the main detected subtype was KPC-2 (n=1 014, 62.17%). 12 drug resistance genes were detected from 2 094 CRKP strains, of which KPC drug resistance genes accounted for more than 50% in 6 regions.Through MLST analysis on 859 CRKP strains, the dominant sequence type was ST11 (659/859, 76.72%), and distributed in 6 regions of China. KPC-2-producing ST11 strain (581/659, 88.16%) was the most prevalence type in China. Conclusion CRKP strains carrying drug resistance genes spread rapidly throughout the country, they have spread all over 6 regions of the country at pre-sent, comprehensive prevention and control measures against CRKP should be established to avoid the outbreak of CRKP in hospitals.