Objective To analyze the clinical characteristics of HIV-negative patients with Talaromycosis, and improve the level of early clinical diagnosis. Methods Clinical data of 8 HIV-negative patients with Talaromycosis in Guangxi Zhuang Autonomous Region Nationality Hospital from January 2016 to November 2020 were analyzed re-trospectively. Results Among the 8 patients, 6 were males and 2 were females, with an average age of 58 years. 6 patients had pulmonary infection, 4 had high fever, 3 had lymphadenopathy, 2 had hepatosplenomegaly, 4 had hypoproteinemia, 3 had bone destruction, and 2 had received anti-tuberculosis treatment. Auxiliary blood routine exa-mination showed that white blood cell of 7 cases increased significantly, 8 cases all had decreased hemoglobin, as well as increased erythrocyte sedimentation rate, C-reactive protein and procalcitonin in varying degrees. Three patients got better and discharged with medicine for maintenance treatment after receiving amphotericin B or itracona-zole treatment, 2 cases were transferred to superior hospital for treatment, 3 patients were with severe multiple organ failure, and voluntarily gave up treatment and discharged. Conclusion There is no specific clinical characteristics among HIV-negative patients with Talaromycosis, which lead to missed diagnosis, misdiagnosis and high mortality, clinicians should pay great attention to it.