Prosthetic joint infection (PJI) is a serious complication after joint arthroplasty. Aggregatibacter aphrophilus (A. aphrophilus) is a fastidious bacteria, which was detected from hip joint puncture fluid after total hip arthroplasty of a patient in a hospital by both metagenomic next-generation sequencing(mNGS) and 16S polymerase chain reaction (16S PCR) detection, the optimal culture of intraoperative specimen was performed according to the detection results, A. aphrophilus was cultured finally and antimicrobial susceptibility testing was performed. After two-stage revision of hip arthroplasty and susceptible antimicrobial treatment, the patient was successfully cured. This paper reports and conducts literature review on a rare case of A. aphrophilus infection after total hip arthroplasty.