Objective To study the cleaning and disinfection methods as well as effect maintenance time of medicine transfer boxes in pharmacy intravenous admixture service (PIVAS), so as to ensure the safety of finished pro-duct infusion. Methods 64 normally used medicine transfer boxes in a hospital were selected, after cleaning and disinfection with medical disinfectant wipes, visual inspection and microbial detection were carried out at 0, 12, 24, 36 and 48 hours respectively, statistical analysis was conducted. Results After 0 hour of cleaning and disinfection, the qualified rates of visual inspection method and microbial detection method were both 100%. Within 24 hours of cleaning and disinfection, there was no liquid residue and visible dirt in the medicine transfer boxes, qualified rate of microbial detection was more than 95%, no pathogenic bacteria were detected, and the maintenance effect was good. With the extension of time, up to 48 hours, qualified rates of visual inspection method and microbial detection method both decreased significantly, bacterial contamination rate was more than 50%, Escherichia coli was detected at the handles of medicine transfer boxes. Contamination rates of handle (57.50%) and outside side(42.50%) of medicine boxes were higher than inside (18.75%), the top three departments of bacterial contamination were neonatology (46.30%), obstetrics (43.48%) and pediatrics (39.13%). Conclusion Cleaning and disinfecting PIVAS medicine transfer boxes every 24 hours by using medical disinfectant wipes can prevent the occurrence of healthcare-associated infection. For clinical departments with large amount of finished product infusion and frequent use of medicine boxes, such as departments of neonatology, obstetrics and pediatrics, the frequency of cleaning and disinfection of medicine transfer boxes should be properly increased, and attention should be paid to the frequently touched sites of medicine transfer boxes, such as the cleaning and disinfection of the handles of medicine boxes.