Objective To analyze the effect of bundle management on ventilator-associated pneumonia (VAP) in department of critical care medicine. Methods From January 2015 to December 2019, 964 patients who were admitted in department of critical care medicine of a hospital and received mechanical ventilation (MV) for more than 48 hours were selected and divided into control group (January 2015-December 2016) and intervention group (January 2017-December 2019) according to admission time and nursing mode. Control group took routine nursing mana- gement measures, intervention group took cycle bundle management mode on the basis of control group. Incidence of VAP, mortality during hospitalization, detection rate of multidrug-resistant organism (MDRO) and infection rate of MDRO in department of critical care medicine were compared between two groups of patients. Results Among 665 patients in intervention group, incidence of VAP was 12.95 ‰, mortality during hospitalization was 4.06%; among 299 patients in control group, incidence of VAP was 25.79 ‰, mortality during hospitalization was 10.37%, incidence of VAP and mortality during hospitalization in intervention group were both lower than those in control group (both P < 0.05). Detection rate of MDRO in intervention group was lower than that in control group (12.77% vs 43.94%, P < 0.05); MDRO infection rates in patients in intervention group of department of critical care medicine was lower than that of control group during the same period (0.52% vs 4.78%, P < 0.05). Conclusion Bundle management measures can reduce the incidence and mortality of VAP in department of critical care medicine, and can also reduce the detection rate and infection rate of MDRO in MV patients, which is worthy of clinical promotion and application.