In view of the high incidence, high drug resistance, high transmission and high mortality of infection in critically ill patients in intensive care unit (ICU), how to conduct scientific prevention and control as well as treatment for drug-resistant bacterial infection has become the focus of global public health and major clinical problem that needs to be solved. Effective prevention of cross transmission and infection of drug-resistant bacteria at common sites of ICU patients is crucial. Focusing on the key issues of infection prevention and control, the standardized infection prevention and control mode for drug-resistant bacteria in ICU was formed through the implementation of key prevention and control measures, including active screening, quarantine, disinfection, patient transport, compliance assessment, etc., so as to effectively reduce the incidence of carbapenem-resistant Gram-negative organism colonization/infection in critically ill patients.