Objective To study the homologous relationship between the carbapenem-resistant Klebsiella pneumoniae (CRKP) in burn patients and the environmental bacterial strains, and provide scientific basis for the prevention and treatment of CRKP infection in burn patients. Methods Patients with CRKP infection from June to December 2020 in burn intensive care unit of a hospital were prospectively monitored. Clinical and environmental specimens were collected. Strains were identified and tested for antimicrobial susceptibility through Compact VITEK 2 automatic microbial analyzer. Homology of the strains were analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Carrying Status of virulence genes and capsular serotype genes were detected. Results A total of 4 burn patients developed CRKP infection, 2 strains were isolated from wound secretion and 2 from blood. 152 environmental specimens were collected, 15 CRKP strains were obtained after bacterial identification and antimicrobial susceptibility test. Cluster analysis through PFGE showed 7 cluster groups A-G. Blood specimens of patient 1, blood and environmental specimens of patient 2, as well as wound secretion and environmental specimens of patient 3 were homologous(all type A). Type A-G strains were analyzed by MLST, and showed that type A-G were ST11, ST35, ST1, ST37, ST2929, ST23, and ST17. Virulence genes of 15 CRKP strains were tested, out of which 5 strains were K57, with ST types of ST35, ST37 and ST17. Conclusion Detection rate of CRKP in the environment of burn patients is high. Strains isolated from environmental specimens were found homologous to that from clinical specimens, indicating that effective control of environmental contamination is conducive to the prevention and control of infection spread. In addition, CRKP exhibits prevalence of clone strains, which may combine with high virulence. More extensive monitoring should be carried out.