Objective To explore the application effect of micro-lecture combined with BOPPPS teaching mode in practical teaching of isolation and precaution technique among medical students. Methods Medical students in grade 2020 from Anhui University of Chinese Medicine and receiving standardized training were selected as the research object, according to cluster random sampling, they were divided into trial group and control group. Control group adopted the teaching mode of traditional multi-media combined with on-site practical demonstration, while trial group adopted the teaching mode of micro-lecture combined with BOPPPS mode. After class, two groups were assessed in theory and practice, and the satisfaction for teaching effect was investigated through anonymous questionnaire. Results A total of 454 medical students were selected as the research object, 240 were in trial group and 214 in control group. The age of trial group and control group were (23.92±3.84) and (23.60±3.21) years old respectively. The score of theoretical examination in trial group was higher than that in control group ([89.44±8.41] points vs [82.76±10.08] points, P < 0.001). The score of practical skill examination in trial group was higher than that in control group ([96.01±1.62] points vs [95.42±2.35] points, P=0.002). The satisfaction of students in trial group in stimulating learning interest and improving autonomous learning ability was higher than that of students in control group (both P < 0.05). Conclusion The teaching mode of micro-lecture combined with BOPPPS can effectively improve students' learning effect, stimulate learning interest and cultivate autonomous learning ability, thus improving teaching quality.