Objective To understand the management of loaner medical instruments and implants in medical institutions in Hubei Province after the implementation of three standards of Regulation for central sterile supply department in hospital (WS 310-2016). Methods According to the requirements of WS 310.1-2016, questionnaire was designed, multi-stage stratified random sampling method was adopted to select secondary and above medical institutions in Hubei Province as the survey objects, the handling status of loaner medical instruments and implants as well as the provision of product instructions was surveyed. Results Among hospitals participating in the survey, 75.76% achieved the centralized supply of loaner medical instruments and implants, 76.36% cleaned and disinfected the loaner medical instruments according to the methods required by the manufacturers, 75.15% met the requirements of sterilization methods and parameters of instructions, and 56.97% returned the used loaner medical instruments to central sterile supply department (CSSD) for re-cleaning and disinfection, only 28.48% of hospitals indicated that they could receive the product instruction of all loaner medical instruments. There were significant differences in the centralized supply, re-cleaning and disinfection after use, as well as the provision of instructions of loaner medical instruments and implants between tertiary hospitals and hospitals below tertiary level (all P < 0.05). Conclusion The cleaning, disinfection, and sterilization of loaner medical instruments and implants in hospitals in Hubei Province do not fully meet the relevant standards, and the proportion of product instructions provided by manufacturers is low, the implementation rate of tertiary hospitals is better than that of hospitals below tertiary le-vel. Medical institutions at all levels should strengthen the management of loaner medical instruments in accordance with relevant regulations to ensure medical quality and patient safety.