Objective To evaluate the effect of setting up full-time healthcare-associated infection (HAI) control staff for implementing on-site supervision in the buffer room of isolation ward. Methods The first-line health care workers (HCWs) who participated in anti-coronavirus disease 2019 (COVID-19) in Zhangjiajie People's Hospital from August to September 2021 were selected as the survey objects, questionnaire about general information, the psychological status of the first-line HCWs after full-time HAI control staff implementing supervision, and effect evaluation after the implementation of supervision by full-time HAI control staff were made, Questionnaire Star was used for survey. Results A total of 227 valid questionnaires were collected. 78.9% of HCWs thought that the on-site supervision of full-time HAI control staff made them feel more secure; 94.7% of HCWs thought that their fear and anxiety had been alleviated in varying degrees; 93.0% of HCWs felt at ease in the first undressing room; 99.6% of HCWs were willing to accept the deficiencies pointed out by full-time HAI control staff on the spot and needed the supervision of full-time HAI control staff; 93.8% of HCWs thought that the environment was better than before; 82.8% of HCWs thought that full-time HAI control staff could prevent the occurrence of non-standard manipulation in time; 98.7% of HCWs thought that in case of emergency, HAI full-time control staff could give correct guidance and help; most HCWs thought that full-time HAI control staff could help them to wear and take off protective clothing. Conclusion The implementation of on-site supervision by HAI full-time staff is conducive to relieving the pressure of first-line HCWs, improving the environment and providing timely help and guidance. In the process of supervision, attention should be paid to women and first-line HCWs who participated in the fight against the epidemic for the first time.