Objective To explore the effect of integrated pre-and in-hospital management on reducing the incidence of aspiration and hypostatic pneumonia in patients with acute ischemic stroke(AIS). Methods 108 AIS patients who were diagnosed and treated in a hospital from March 2020 to October 2021 were selected as the research objects, according to the different intervention time and management modes, 55 AIS patients who received routine nursing operation in the hospital from March to December 2020 were as control group, and 53 AIS patients who received integrated pre-and in-hospital management from January to October 2021 were as intervention group, incidence of aspiration and hypostatic pneumonia, average hospitalization time, patient satisfaction of two groups of AIS patients, as well as scores in the examination of infection prevention and control, airway management knowledge, compliance rate of hand hygiene of doctors and nurses in two patients' groups were compared. Results Incidences of aspiration and hypostatic pneumonia in AIS patients in intervention group were 9.43% and 3.77% respectively, which were both lower than those in control group (25.45% and 14.55% respectively); the average hospitalization time of intervention group was shorter than that of control group ([10.79±5.35] days vs ([13.69±6.84] days), patients' satisfaction was higher than that of control group (96.23% vs 81.82%) (all P < 0.05). The scores of knowledge on healthcare-associated infection (HAI) prevention and control, airway management as well as hand hygiene compliance rate of doctors and nurses in patients' intervention group were higher than those in control group (all P < 0.001). Conclusion Early intervention in AIS patients with integrated pre-and in-hospital management mode can reduce the incidence of aspiration and hypostatic pneumonia, effectively shorten hospitalization time of patients, improve the satisfaction of patients, enhance the awareness of infection prevention and control of health care workers, and improve prevention and control level of HAI, which is worthy of clinical promotion and application.