Objective To investigate the medical students' education needs and teachers' suggestion on course design of healthcare -associated infection (HAI) prevention and control. Methods Medical students' and teachers' education needs and opinions on course design of HAI prevention and control in 10 medical universities in 5 regions of China was conducted questionnaire survey, the course design scheme was demonstrated by referring to course design steps of Taba curriculum model. Results Among 584 students, only 11.8% (n=69) believed that the current education on HAI prevention and control could fully meet their needs, and the top four in demand scores were hand hygiene, disinfection and sterilization isolation, as well as introduction to HAI and occupational protection. Among 352 teachers, 72.7% (n=256) suggested setting up compulsory courses; 86.4% (n=304) chose 8-24 class hours in total, 12.5% (n=44) chose more than 24 class hours, the surveyed teachers suggested that the average total class hours of 10 chapters be 19.5; it is required to master 13 items and be familiar with 9 items of knowledge and skills; 86.4% (n=304) of teachers suggested that the ratio of theoretical and probation hours should be 1 ∶0.5 or more; 53.4% (n=188) of teachers suggested taking the form of theoretical courses+group probation (including skill manipulation)+online learning; 63.7% (n=224) of teachers suggested that the teaching team should be composed of teachers from HAI management department+infectious diseases department+nursing department. Conclusion At present, education of HAI prevention and control for medical students is far from fully meeting the needs, so it is necessary to have independent courses, it is suggested to set up 16-24 class hours of compulsory courses, integrate theory with practice, implement diversified teaching for multidisciplinary teachers, and assess according to the compulsory courses.